rnase free sirna duplex resuspension buffer (OriGene)

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OriGene rnase free sirna duplex resuspension buffer
Rnase Free Sirna Duplex Resuspension Buffer, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/duplex+buffer/siRNA+Related+Product/pm42849439-230-196-201
Average 94 stars, based on 18 article reviews

rnase free sirna duplex resuspension buffer - by Bioz Stars, 2026-10

94/100 stars

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Negative Control:

Article Title: Impacts of Arsenic on Rad18 and Translesion Synthesis
Article Snippet: Sodium arsenite (>99%) was purchased from Fluka Chemie (Buchs, Switzerland); zinc chloride (>98%) from Sigma (St. Louis, MO); and 16% Formaldehyde Solution from Thermo Scientific (Waltham, MA). .. The Rad18 Human siRNA Oligo Duplex kit was obtained from Origene (Rockville, MD) and contained 3 unique Rad18 27mer siRNA duplexes A-C #SR311213 (Locus ID 56852; RefSeq NM_020165 ), Trilencer-27 Universal Scrambled Negative Control siRNA Duplex #SR30004, and RNAse free siRNA Duplex Resuspension Buffer. .. The Trilencer-27 Fluorescent-labeled transfection control siRNA duplex #SR30002 and siTran 2.0 siRNA Transfection Reagent kit #TT320001 containing siTran 2.0 Transfection Reagent and 5x Transfection Buffer were purchased from Origene. siRNA Duplex Resuspension Buffer was added to siRNA duplexes to a final concentration of 10 μM according to manufacturer’s instructions and aliquots frozen at −20 °C.

Article Title: Rlip overexpression reduces oxidative stress and mitochondrial dysfunction in Alzheimer's disease: Mechanistic insights.
Article Snippet: Alzheimer’s disease (AD) is a neurodegenerative disease that affects a large proportion of the aging population.. RalBP1 (Rlip) is a stress-activated protein that plays a crucial role in oxidative stress and mitochondrial dysfunction in aging and neurodegenerative diseases but its precise role in the progression of AD is unclear.. The purpose of our study is to understand the role of Rlip in the progression and pathogenesis of AD in mutant APP/ amyloid beta (Aβ)-expressed mouse primary hippocampal (HT22) hippocampal neurons.

Article Title: Spinal dopaminergic D 1 and D 5 receptors contribute to reserpine-induced fibromyalgia-like pain in rats.
Article Snippet: Fibromyalgia is a complex pain syndrome without a precise etiology.. Reduced monoamines levels in serum and cerebrospinal fluid in fibromyalgia patients has been reported and could lead to a dysfunction of descending pain modulatory system producing the painful syndrome.. This study evaluated the role of D1-like dopamine receptors in the reserpine-induced fibromyalgia-like pain model in female Wistar rats.

other:

Article Title: Glucocorticoids regulate lipid mediator networks by reciprocal modulation of 15-lipoxygenase isoforms affecting inflammation resolution
Article Snippet: Trilencer-27 universal scrambled negative control siRNA (Origene, SR30004) represents the non-target control (non-T).

Article Title: Immunoregulatory gene GIMAP6 suppresses lethal atherosclerotic vasculopathy and ischemic heart failure.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER SuperSignal West Dura Extended Duration Substrate Thermo Fisher Scientific Cat# 34076 Restore Western Blot Stripping Buffer Thermo Fisher Scientific Cat# 21063 Cycloheximide GlpBio Cat# GC17198 Recombinant mouse M-CSF GenScript Cat# Z02930 Recombinant Mouse TNF-α (carrier-free) BioLegend Cat# 575202 Matrigel Growth Factor Reduced Basement Membrane Matrix Corning Life Sciences Cat# 356231 Endothelial cell growth supplement Sigma-Aldrich Cat# E2759 Heparin sodium salt Sigma-Aldrich Cat# H3393 Dispase I Sigma-Aldrich Cat# D4818 Fibronectin Sigma-Aldrich Cat# F1141 Sulfosuccinimidyl oleate Cayman Chemical Cat# 11211 HEPES sodium salt Sigma-Aldrich Cat# H7006 ITS Liquid Media Supplement (100×) Sigma-Aldrich Cat# I3146 BODIPY FL C16 Thermo Fisher Scientific Cat# D3821 BODIPY 493/503 GLPbio Technology Inc Cat# GC42959 MitoSOX Red mitochondrial superoxide indicator Thermo Fisher Scientific Cat# M36008 MitoTEMPO MCE Cat# HY-112879 N-acetyl-L-cysteine Millipore Sigma Cat# A9165-5G GW9662 MCE Cat# HY-16578 GW3965 MCE Cat# HY-10627 Oxidized Low-density Lipoprotein (OxLDL) Thermo Fisher Scientific Cat# L34357 Pierce Dimethylsulfoxide (DMSO), Sequencing Grade Thermo Fisher Scientific Cat# 20688 eBioscience Fixable Viability Dye eFluor 450 Thermo Fisher Scientific Cat# 65-0863-14 Human TruStain FcX BioLegend Cat# 422302 Nuclease-Free Duplex Buffer Integrated DNA Technologies Cat# 11-01-03-01 ATTO550-labeled tracrRNA Integrated DNA Technologies Cat# 1075927 Cas9 protein MacroLab, UC Berkeley N/A RNase-free siRNA duplex resuspension buffer OriGene Cat# SR30005 Non-targeting control siRNA OriGene Cat# SR30004 GIMAP6 siRNA pool OriGene Cat# SR318628 SPLASH Lipidomix Mass Spec Standard Avanti Polar Lipids Cat# 330707 Methanol, LC-MS grade Fisher Scientific Cat# A456 Isopropanol, LC-MS grade Fisher Scientific Cat# A461 Acetonitrile, LC-MS grade Fisher Scientific Cat# A955 Water, LC-MS grade Fisher Scientific Cat# W6212 Chloroform, LC-MS grade Sigma-Aldrich Cat# 650471-1L Methyl tert-butyl ether Honeywell Cat# 34875-1L Butylated hydroxytoluene Sigma-Aldrich Cat# B1253-500G Ammonium formate Thermo Fisher Scientific Cat# 014517.30 Ammonium carbonate Thermo Fisher Scientific Cat# 196655000 Formic acid, LC-MS grade Thermo Fisher Scientific Cat# 28905 Medronic acid MCE Cat# HY-108309 Sodium Pyruvate Solution MCE Cat# HY-K3024 D-glucose Sigma-Aldrich Cat# G8270 (Continued on next page) e3 Cell 189, 6792–6809.e1–e21, October 15, 2026

Transfection:

Article Title: Cancer-Associated Fibroblast Induces Acinar-to-Ductal Cell Transdifferentiation and Pancreatic Cancer Initiation Via LAMA5/ITGA4 Axis.
Article Snippet: Cancer-Associated Fibroblast Induces Acinar-to-Ductal Cell Transdifferentiation and Pancreatic Cancer Initiation Via LAMA5/ITGA4 Axis Seema Parte, Annant B. Kaur, Rama Krishna Nimmakayala, Ayoola O. Ogunleye, Ramakanth Chirravuri, Raghupathy Vengoji, Frank Leon, Palanisamy Nallasamy, Sanchita Rauth, Zahraa Wajih Alsafwani, Subodh Lele, Jesse L. Cox, Ishfaq Bhat, Shailender Singh, Surinder K. Batra, and Moorthy P. Ponnusamy

Incubation:

Protease Inhibitor:

Article Title: MARCH7-mediated ubiquitination decreases the solubility of ATG14 to inhibit autophagy.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-ATG14 MBL Cat#PD2600 Anti-ATG14 ABclonal Cat#A7526; RRID:AB_2768053 Anti-MARCH7 ABclonal Cat#A16308; RRID:AB_2770291 Anti-MARCH7 Santa Cruz Biotechnology Cat#sc-166945; RRID:AB_10609950 Anti-SQSTM1/p62 Abcam Cat#ab56416; RRID:AB_945626 Anti-MAP1LC3B/LC3B Proteintech Cat#14600-1-AP; RRID:AB_2137737) Anti-STX17 Proteintech Cat#17815-1-AP; RRID:AB_2255542 Anti-Flag Sigma-Aldrich Cat#F1804; RRID:AB_262044 Anti-Flag Sigma-Aldrich Cat#F7425; RRID:AB_439687 Anti-mCherry Abcam Cat#ab167453; RRID:AB_2571870 Anti-Myc Proteintech Cat#16286-1-AP; RRID:AB_11182162 Anti-HA Sigma-Aldrich Cat#H6908; RRID:AB_260070 Anti-Strep II Tag Abcepta Cat#AM8623b Anti-GST Santa Cruz Biotechnology Cat#sc-138; RRID:AB_627677 Anti-GAPDH Santa Cruz Biotechnology Cat#sc-47724; RRID:AB_627678 Anti-Ubiquitin (P4D1) Santa Cruz Biotechnology Cat#sc-8017 AC; RRID:AB_2762364 Anti-BECN1 Proteintech Cat#11306-1-AP; RRID:AB_2259061 Anti-PIK3C3 Proteintech Cat#12452-1-AP; RRID:AB_2299709 Anti-PIK3R4 GeneTex Cat#GTX132466; RRID:AB_2886653 Rabbit IgG Bethyl Cat#P120-101; RRID:AB_479829 Alexa Flour 647-conjugated anti-rabbit IgG Thermo Fisher Scientific Cat#A32733; RRID:AB_2633282 Alexa Flour 647-conjugated anti-mouse IgG Thermo Fisher Scientific Cat#A32728; RRID:AB_2633277 Alexa Flour 488-conjugated anti-rabbit IgG Thermo Fisher Scientific Cat#A-11008; RRID:AB_143165 Alexa Flour 488-conjugated anti-mouse IgG Thermo Fisher Scientific Cat#A-11001; RRID:AB_2534069 Alexa Flour 568-conjugated anti-rabbit IgG Thermo Fisher Scientific Cat#A-11011; RRID:AB_143157 Alexa Flour 568-conjugated anti-mouse IgG Thermo Fisher Scientific Cat#A-11004; RRID:AB_2534072 Chemicals, peptides, and recombinant proteins Bafilomycin A1 MedChemExpress Cat#HY-100558 Chloroquine (CQ) Sigma-Aldrich Cat#C6628 Doxycycline (DOX) MedChemExpress Cat#N0565B MG132 Invivogen Cat#tlrl-mg132 Hexadimethrine bromide MedChemExpress Cat#HY-112735 Lipofectamine 2000 Thermo Fisher Scientific Cat#11668019 LipofectamineTM RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat#13778150 Phanta Max Super-Fidelity DNA polymerase Vazyme Cat#P505-d2 Puromycin MedChemExpress Cat#HY-B1743A Hygromycin MedChemExpress Cat#HY-B0490 Penicillin Sigma-Aldrich Cat#P3032 Streptomycin Gibco Cat#11860-038 Polyethylenimine (PEI) Polysciences Cat#24765 2-Mercaptoethanol Sigma-Aldrich Cat#M3148 Ampicillin Sigma-Aldrich Cat#A9518 Kanamycin Mdbio Cat#K001 (Continued on next page) Cell Reports 42, 113045, September 26, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Isopropyl b-D-thiogalactoside (IPTG) MedChemExpress Cat#HY-15921 Phenylmethyl sulfonyl fluoride (PMSF) Sangon Biotech Cat#A610425 DL-Dithiothreitol (DTT) Sigma-Aldrich Cat#D9779 Glycerol Sigma-Aldrich Cat#V900122 Glutathione Resin Genscript Cat#L00206 Imidazole Sigma-Aldrich Cat#V900153 Urea Sigma-Aldrich Cat#V90011 Biotin Sigma-Aldrich Cat#V900418 Protease Inhibitor Cocktail Roche Cat#4693132001 Flag M2 affinity agarose beads Sigma-Aldrich Cat#A2220 c-Myc beads Thermo Fisher Scientific Cat#88843 Strep-Tactin beads IBA Life Sciences Cat#2-5030-025 Ni-NTA agarose QIAGEN Cat#30230 Protein A beads BIO-RAD Cat#161-4013 4% formaldehyde buffer DeweiBio Cat#D60714 PBS Origene CatZLI-9063 Triton X-100 Sigma-Aldrich Cat#T9284 40-6-diamidino-2-phenylindole (DAPI) Cell Signaling Technology Cat#4083S Trypsin Promega Cat#V5280 Trifluoroacetic acid (TFA) Thermo Fisher Scientific Cat#85183 ImperialTM Protein Stain Thermo Fisher Scientific Cat#UA275057 Ammonium bicarbonate (ABC) Sigma-Aldrich Cat#09830 Iodoacetamide alkyne (IAA) Thermo Fisher Scientific Cat#I10189 Chloroacetamide (CAA) Sigma-Aldrich Cat#C0267 Acetonitrile Thermo Fisher Scientific Cat#51101 Bis(sulfosuccinimidyl) glutarate (BS2 G) Thermo Fisher Scientific Cat#21610 4%–15% Mini-PROTEAN TGX Gel BIO-RAD Cat#4561084 C18 Empore Cat#6683-U DMEM Gibco Cat#C11995500BT DMEM/F12 Gibco Cat#11330-032 Expi293F culture medium Thermo Fisher Scientific Cat#A1435101 Earle’s balanced salt solution (EBSS) HyClone Cat#SH30029.02 GSH Sigma-Aldrich Cat#V900456 Ni Sepharose 6 Fast Flow GE Healthcare Cat#17531803 Superdex 75 increase 10/300 GL Cytiva Cat#29148721 10,000 MWCO Amicon Ultra 15 Centrifugal Filter Millipore Cat#UFC901024 30,000 MWCO Amicon Ultra 15 Centrifugal Filter Millipore Cat#UFC903024 Twinstrep-Flag-ATG14 This study N/A GST-MARCH7-D2 This study N/A His-HA-Ubiquitin This study N/A USP2 This study N/A OTUB1 This study N/A AMSH This study N/A Cezanne This study N/A OTULIN This study N/A OYUD3 This study N/A YOD1 This study N/A TRABID This study N/A (Continued on next page) 16 Cell Reports 42, 113045, September 26, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays BCA assay kit Thermo Fisher Scientific Cat#23225 Subcellular Protein Fractionation Kit for Cultured Cells Thermo Fisher Scientific Cat#78840 Lysosomal Intracellular Activity Assay Abcam Cat#ab234622 Deposited data Mass spectrometry raw data This study PRIDE: PXD042608, PRIDE: PXD042609 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216; RRID:CVCL_0063 U-2 OS ATCC Cat#HTB-96; RRID:CVCL_0042 HeLa ATCC Cat#CCL-2; RRID:CVCL_0030 SH-SY5Y ATCC Cat#CRL-2266; RRID:CVCL_0019 Expi293F Thermo Fisher Scientific Cat#A14635 U-2 OS Flag-ATG14 This study N/A HeLa inducible_shMARCH7 This study N/A HeLa inducible_shATG14-1 This study N/A HeLa inducible_shATG14-2 This study N/A HeLa MARCH7 KO-1 This study N/A HeLa MARCH7 KO-2 This study N/A HeLa MARCH7 KO-3 This study N/A HEK293T MARCH7 KO This study N/A HEK293T ATG14 KO This study N/A Oligonucleotides ATG14 cloning primers: 50-ATGGCGTCTCCCAG TGGGAAGG-30 and 50-ACGGTGTCCAGTGTAA GCTTTAAACC-30 This study N/A MARCH7 cloning primers: 50-ATGGAGTCTAAA CCTTCAAGGATTCCA-30 and 50-GGCAATATCA AATGTCCTGTTATGGT-30 This study N/A ATG14-D1 cloning primers: 50-ATGGCGTCTCC CAGTGGGAAGG-30 and 50-gcggccgtcgaagtaga cgaa-30 This study N/A ATG14-D2 cloning primers: 50-gaccgggagaggt ttatcgacaagaa-30 and 50-ggtctttttttctaccaggtc accaa-30 This study N/A ATG14-D3 cloning primers: 50-attgacttaagaagt cattatgagcgtct-30 and 50-ACGGTGTCCAGTGT AAGCTTTAAACC-30 This study N/A MARCH7-D1 cloning primers: 50-ATGGAGTCT AAACCTTCAAGGATTCCA-30 and 50-agatctaga tgatgtatcagaggtaggt-30 This study N/A MARCH7-D2 cloning primers: 50-catatttttaga agagaatcaaatgaagt-30 and 50-GGCAATATCA AATGTCCTGTTATGGT-30 This study N/A MARCH7-DE3 cloning primers: 50-ctggagga ttttgatattcatgaactac-30, 50-gtcctctaaaaggag gctctcttttat-30 This study N/A MARCH7-WI cloning primers: 50-GCttgtcaaa tggcagctgca-30, 50-tctacataagtcaccttcttc-30, 50-gcgttacaggccaaaattaactc-30 and 50-ctttttc atacagtcttggtggac-30 This study N/A (Continued on next page) Cell Reports 42, 113045, September 26, 2023 17

Staining:

Control:

Article Title: Overexpression of the erythropoietin receptor is associated with upregulation of tubulin beta 6 and paclitaxel resistance in ovarian adenocarcinoma cells.
Article Snippet: .. Both the target and control siRNAs were dissolved in RNase-free siRNA duplex re-suspension buffer (OriGene Technologies, Inc.; #SR30005) according to the manufacturer’s instructions. ..

Review